ABSTRACT
Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared
Methods: Glucose uptake measurement was performed using 2-[N-[7-nitrobenzin-2-oxa-1, 3- diazol-4-yl] amino]-2-deoxyglucose [2-NBDG] and Omega FluoStar plate reader. Incubation of cells [10[4]/ml, in 24 well plate at 37 [degree sign]C in 5% CO[2] / air] with three phytoestrogens at concentration of 10[-4] M to 10[-9]M in two studies mood, short term treatment [one hour] and long term treatment [24 hours] was tested
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 micro M, which stimulated uptake about three-fold compared with control. Longer treatments with 10[-4]M to 10[-6] M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA
ABSTRACT
Introduction: Cognitive disorders are one of geriatrics outcomes that can cause a vast spectrum of disorders in older people. This change in cognitive function may see as an early sign before behavioral manifestations. Thus early diagnosis in high risk older people can be an important step in prevention in cognitive disorders and disabilities in this population. The purpose of this study is assessment of older people cognitive status based on demographic characteristics
Methods: This study is a cross-sectional, descriptive study that was conducted in 1389-1388 in Rasht. 194 older people that retired form governmental organization participate in that and were selected in regular randomized sampling. The instrument for gathering data was Mini Mental State Examination [MMSE] that has 5 parts in orientation, data recording, concentration and calculation, remembering and verbal skills. Data were assessed after gathering and were analyzed by SPSS v.16 and Kruskal Valise, Man Whitney U and Spear man statistical test
Results: The findings showed that among 194 older people 70.6 percent were man and 29.4 percent were women. 91 percent of illiteracy older people had normal cognitive status, 7.5 percent of them had mild cognitive disorder and 1.5 percent of them had moderate cognitive disorder. Among graduate persons, 82 percent had normal cognitive status, 17.3 percent had mild cognitive disorder and 0.8 percent had moderate cognitive disorder. Among demographic character [age, sex, marries, education and morbidity] cognitive status has a significant correlation with age [P<0.03], marriage status [P<0.002] and education [P<0.0001]
Conclusion: The study showed that cognitive status assessment by health care professionals can help in detecting the high risk patients and promote their caring. Moreover it can help to potential senilement health care and help to delay in starting and progressing cognitive disorders among older people